{"id":4243,"date":"2023-11-07T16:01:30","date_gmt":"2023-11-07T16:01:30","guid":{"rendered":"https:\/\/plassmat.cnrs-imn.fr\/techniques-and-equipment\/sem-fib-microscopies\/zeiss-crossbeam-550l-fib\/"},"modified":"2026-10-13T09:05:04","modified_gmt":"2026-10-13T09:05:04","slug":"zeiss-crossbeam-550l-fib","status":"publish","type":"page","link":"https:\/\/plassmat.cnrs-imn.fr\/en\/techniques-and-equipment\/sem-fib-microscopies\/zeiss-crossbeam-550l-fib\/","title":{"rendered":"Zeiss Crossbeam 550L FIB"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"4243\" class=\"elementor elementor-4243 elementor-1710\" data-elementor-post-type=\"page\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-eb9f2c7 elementor-reverse-mobile elementor-reverse-tablet elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-invisible\" data-id=\"eb9f2c7\" 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class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"512\" height=\"512\" src=\"https:\/\/plassmat.cnrs-imn.fr\/wp-content\/uploads\/2023\/08\/cropped-favicon.png\" class=\"attachment-full size-full wp-image-3533\" alt=\"\" srcset=\"https:\/\/plassmat.cnrs-imn.fr\/wp-content\/uploads\/2023\/08\/cropped-favicon.png 512w, https:\/\/plassmat.cnrs-imn.fr\/wp-content\/uploads\/2023\/08\/cropped-favicon-300x300.png 300w, https:\/\/plassmat.cnrs-imn.fr\/wp-content\/uploads\/2023\/08\/cropped-favicon-150x150.png 150w, https:\/\/plassmat.cnrs-imn.fr\/wp-content\/uploads\/2023\/08\/cropped-favicon-270x270.png 270w, https:\/\/plassmat.cnrs-imn.fr\/wp-content\/uploads\/2023\/08\/cropped-favicon-192x192.png 192w, https:\/\/plassmat.cnrs-imn.fr\/wp-content\/uploads\/2023\/08\/cropped-favicon-180x180.png 180w, https:\/\/plassmat.cnrs-imn.fr\/wp-content\/uploads\/2023\/08\/cropped-favicon-32x32.png 32w\" sizes=\"(max-width: 512px) 100vw, 512px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-dea557c elementor-widget__width-auto elementor-widget-mobile__width-inherit elementor-widget elementor-widget-heading\" data-id=\"dea557c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Technical specifications<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e6463dd elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"e6463dd\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8eb0179 elementor-widget elementor-widget-text-editor\" data-id=\"8eb0179\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>The instrument is equipped with an Oxford EDS detector for chemical element analysis and an EBSD detector for characterizing crystal orientation phases. Both techniques can be used in parallel with 3D data acquisition.<br\/>A sample cryogenization system allows for work at very low temperatures on samples sensitive to the ambient atmosphere under the beam (Quorum). It includes a cold transfer system between the instrument and a controlled-atmosphere glove box.<br\/>The microscope is also coupled to a Raman spectrometer (Renishaw).<br\/>A vacuum transfer system is provided between a glove box and the microscope.  <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<div class=\"elementor-element elementor-element-2afff59 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"2afff59\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-255c559 elementor-reverse-mobile elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"255c559\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-ec3c30b\" data-id=\"ec3c30b\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-95c528b elementor-widget elementor-widget-text-editor\" data-id=\"95c528b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Extraction of a slide for TEM microscopy<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-cdee1d8 elementor-widget elementor-widget-text-editor\" data-id=\"cdee1d8\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>To work on a bulk material using <a href=\"https:\/\/plassmat.cnrs-imn.fr\/en\/techniques-and-equipment\/tem-microscopy\/\">transmission electron microscopy<\/a>, it must be thin enough for the electron beam to pass through it, and for the information transmitted not to be degraded by the thickness of the observed zone. Before the advent of the FIB, this was a complicated task performed by mechanical or chemical means, or by exposure to an ion beam. These methods are very approximate to achieve a correct thickness and, most importantly, to target a very specific area in a block, with the naked eye or by optical means.  <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-221de70 elementor-widget elementor-widget-text-editor\" data-id=\"221de70\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>The FIB machining precision now makes it possible to cut a material and extract a section a hundred nanometers thick. The extraction location is pinpointed on the sample using electronic imaging, allowing the removal of a section from the exact spot intended for analysis. First, a thick section is removed from the material. It is then welded onto a nanomanipulator by ion-beam organometallic deposition (platinum), transported and welded onto a copper grid compatible with the MET <a href=\"https:\/\/plassmat.cnrs-imn.fr\/en\/techniques-and-equipment\/tem-microscopy\/nantthemis\/\">Nant&#8217;Themis<\/a>object holder. Final thinning is achieved with the ion beam.    <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<div class=\"elementor-element elementor-element-be331e9 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"be331e9\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-46ca642 elementor-widget elementor-widget-text-editor\" data-id=\"46ca642\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Observation of a section<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-382fdf0 elementor-widget elementor-widget-text-editor\" data-id=\"382fdf0\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>In most abrasion operations, the sample surface is perpendicular to the ion beam and inclined at 54\u00b0 to the electron beam. This arrangement enables the electron column to be used to observe the sidewall of an ion beam trench, provided that the observation is not hindered by the opposite wall. By choosing the shape of the hole to open up the perspective for the electron column (an opening that can be compared to that of a slope to access an underground garage), we make it possible to observe a cut in the depth of the sample. This is done exactly where needed, and without any prior sample preparation (whereas, without an ion column, the sample should be cut and polished before being fed into the machine).   <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a3b3ebd elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"a3b3ebd\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d314b72 elementor-widget elementor-widget-text-editor\" data-id=\"d314b72\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>3D reconstruction<\/strong><\/p><p><strong> <\/strong><\/p><p>The abrasion capacity of the ion beam is precise enough to remove a very thin layer of material (down to about ten nanometers). By repeating this operation a large number of times over the same surface, we explore a volume of the sample whose depth is proportional to the number of layers removed. By taking an electronic image of the surface each time a layer is removed, we get a collection of images. Stacked one on top of the other, these images reflect the internal morphology of the abraded volume.  <\/p><p>3D reconstruction software processes this collection of images to obtain an in-depth visualization of the sample. The software can be used to extract numerical data (e.g. porosity or volume occupied by a phase) and generate graphical representations. <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-adbabed elementor-widget__width-initial elementor-widget elementor-widget-video\" data-id=\"adbabed\" data-element_type=\"widget\" data-e-type=\"widget\" data-settings=\"{&quot;video_type&quot;:&quot;hosted&quot;,&quot;controls&quot;:&quot;yes&quot;}\" data-widget_type=\"video.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"e-hosted-video elementor-wrapper elementor-open-inline\">\n\t\t\t\t\t<video class=\"elementor-video\" src=\"https:\/\/plassmat.cnrs-imn.fr\/wp-content\/uploads\/2023\/12\/3d_PES_JOLI_FIN.mp4\" controls=\"\" preload=\"metadata\" controlsList=\"nodownload\"><\/video>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-620a171 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"620a171\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-abf79d3 elementor-widget elementor-widget-text-editor\" data-id=\"abf79d3\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Observation and cold machining<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-0b60d1a elementor-widget elementor-widget-text-editor\" data-id=\"0b60d1a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Some materials are damaged by the beam or by the vacuum in the column of scanning electron microscopes. This mainly concerns samples containing water, but also materials referred to as &#8220;fragile&#8221;. The double-beam microscope is equipped to observe these materials at cold temperature, thanks to a QUORUM system that freezes a sample in liquid nitrogen before introducing it into a preparation chamber, connected to the microscope by an airlock.  <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-bb1c401 elementor-widget elementor-widget-text-editor\" data-id=\"bb1c401\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>A system of circulating cooled nitrogen gas in a liquid nitrogen dewar maintains the sample at -140\u00b0C in the preparation chamber, under the protection of a trap cooled to -170\u00b0C (to trap residual contaminants). The chamber is equipped with knives to break the sample and then metallize it after possible sublimation. The sample is then introduced into the microscope for observation on a support cooled in the same way as in the preparation chamber, and similarly protected by a cold trap.  <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-18a81bc elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"18a81bc\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-947daaa elementor-reverse-mobile elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"947daaa\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-edbf4a2\" data-id=\"edbf4a2\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-7d993c0 elementor-widget elementor-widget-text-editor\" data-id=\"7d993c0\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>SEM\/FIB-Raman Coupling<\/strong><\/p><p> <\/p><p>Due to limitations in optical resolution, standard commercial Raman spectrometers are difficult to use for detecting and studying objects smaller than the wavelength of visible light. This limitation has been overcome by coupling a Renishaw InVia Raman spectrometer to the SEM\/FIB via an optical fiber. A laser beam is thus guided into the SEM chamber through an arm positioned between the sample and the electron gun. Thanks to an aperture in the parabolic mirror of the arm, the electron gun can image the sample\u2019s surface at the exact location where the Raman measurement is taken. Unlike other similar devices, these two measurements are thus performed without moving the sample, allowing for a perfect correlation between electron microscopy and the physicochemical identification provided by Raman spectroscopy.    <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-102f2ce elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"102f2ce\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f10d74c elementor-widget elementor-widget-text-editor\" data-id=\"f10d74c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Example of the Use of SEM\/FIB-Raman Coupling<\/strong><\/p><p> <\/p><p>Coupling with the FIB section of the SEM allows for in-situ Raman analysis under controlled temperature conditions. For example, we were able to effectively evaluate the accuracy of cryofixation methods. Analyses were thus performed on highly hydrated calcium phosphate cement that had been frozen by immersion, as well as on a gelatin-based composite. After cutting a trench of defined thickness using the FIB, Raman analyses were performed at a specific depth within the frozen material. The results show that the Raman signal is sensitive to changes in the molecular structures of the aqueous phase and can be used to examine the depth of vitreous ice in frozen samples.    <\/p><p> <\/p><p>Read the post:<\/p><p><a href=\"https:\/\/pubs.acs.org\/ancham\/article\/94\/23\/8120\/1649289\/Application-of-a-Cryo-FIB-SEM-Raman-Instrument-to\">https:\/\/pubs.acs.org\/ancham\/article\/94\/23\/8120\/1649289\/Application-of-a-Cryo-FIB-SEM-Raman-Instrument-to<\/a><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-f0c40cc elementor-hidden-mobile\" data-id=\"f0c40cc\" data-element_type=\"column\" data-e-type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;gradient&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-a8b8101 elementor-widget elementor-widget-spacer\" data-id=\"a8b8101\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-7db448cf elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"7db448cf\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-1b101656\" data-id=\"1b101656\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-747d020f elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"747d020f\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-3c9764c8\" data-id=\"3c9764c8\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-5751252c elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-invisible\" data-id=\"5751252c\" data-element_type=\"section\" data-e-type=\"section\" data-settings=\"{&quot;animation&quot;:&quot;slideInUp&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-10c177e7\" data-id=\"10c177e7\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6bd1bc0a elementor-widget__width-auto elementor-widget-mobile__width-inherit elementor-widget elementor-widget-image\" data-id=\"6bd1bc0a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"512\" height=\"512\" src=\"https:\/\/plassmat.cnrs-imn.fr\/wp-content\/uploads\/2023\/08\/cropped-favicon.png\" class=\"attachment-full size-full wp-image-3533\" alt=\"\" srcset=\"https:\/\/plassmat.cnrs-imn.fr\/wp-content\/uploads\/2023\/08\/cropped-favicon.png 512w, https:\/\/plassmat.cnrs-imn.fr\/wp-content\/uploads\/2023\/08\/cropped-favicon-300x300.png 300w, https:\/\/plassmat.cnrs-imn.fr\/wp-content\/uploads\/2023\/08\/cropped-favicon-150x150.png 150w, https:\/\/plassmat.cnrs-imn.fr\/wp-content\/uploads\/2023\/08\/cropped-favicon-270x270.png 270w, https:\/\/plassmat.cnrs-imn.fr\/wp-content\/uploads\/2023\/08\/cropped-favicon-192x192.png 192w, https:\/\/plassmat.cnrs-imn.fr\/wp-content\/uploads\/2023\/08\/cropped-favicon-180x180.png 180w, https:\/\/plassmat.cnrs-imn.fr\/wp-content\/uploads\/2023\/08\/cropped-favicon-32x32.png 32w\" sizes=\"(max-width: 512px) 100vw, 512px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4f01280d elementor-widget__width-auto elementor-widget-mobile__width-inherit elementor-widget elementor-widget-heading\" data-id=\"4f01280d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Other SEM microscopy equipment<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-a7a3e26 elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-invisible\" data-id=\"a7a3e26\" data-element_type=\"section\" data-e-type=\"section\" data-settings=\"{&quot;animation&quot;:&quot;slideInUp&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 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class=\"elementor-element elementor-element-84ec02c elementor-widget__width-auto elementor-widget elementor-widget-heading\" data-id=\"84ec02c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Would you like to find out more?\n<br>Need some advice?\n<br>Contact us!<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-37f822e\" data-id=\"37f822e\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-18632d5 elementor-align-left elementor-mobile-align-justify elementor-tablet-align-center elementor-widget elementor-widget-button\" data-id=\"18632d5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"mailto:plassmat@cnrs-imn.fr\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">plassmat@cnrs-imn.fr<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-5953530 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"5953530\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-42d2c62\" data-id=\"42d2c62\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-75f6745 elementor-widget elementor-widget-text-editor\" data-id=\"75f6745\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>The FIB was financed by the 2015-2020 State-Region Plan Contract (CPER).<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-316284f elementor-widget elementor-widget-image\" data-id=\"316284f\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"1954\" height=\"268\" src=\"https:\/\/plassmat.cnrs-imn.fr\/wp-content\/uploads\/2024\/01\/financement-accueil-3.png\" class=\"attachment-full size-full wp-image-3735\" alt=\"State, Pays de la Loire Region, Nantes M\u00e9tropole, ERDF and CNRS\" srcset=\"https:\/\/plassmat.cnrs-imn.fr\/wp-content\/uploads\/2024\/01\/financement-accueil-3.png 1954w, https:\/\/plassmat.cnrs-imn.fr\/wp-content\/uploads\/2024\/01\/financement-accueil-3-300x41.png 300w, https:\/\/plassmat.cnrs-imn.fr\/wp-content\/uploads\/2024\/01\/financement-accueil-3-1024x140.png 1024w, https:\/\/plassmat.cnrs-imn.fr\/wp-content\/uploads\/2024\/01\/financement-accueil-3-768x105.png 768w, https:\/\/plassmat.cnrs-imn.fr\/wp-content\/uploads\/2024\/01\/financement-accueil-3-1536x211.png 1536w\" sizes=\"(max-width: 1954px) 100vw, 1954px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>The instrument is equipped with an EDS detector for the analysis of chemical elements, and an EBSD detector to characterize crystalline orientation phases. Both techniques can be used in parallel with 3D acquisition. <\/p>\n","protected":false},"author":1,"featured_media":3663,"parent":4233,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-4243","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.6 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Zeiss Crossbeam 550L FIB - PLASSMAT<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/plassmat.cnrs-imn.fr\/en\/techniques-and-equipment\/sem-fib-microscopies\/zeiss-crossbeam-550l-fib\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Zeiss Crossbeam 550L FIB - PLASSMAT\" \/>\n<meta property=\"og:description\" content=\"The instrument is equipped with an EDS detector for the analysis of chemical elements, and an EBSD detector to characterize crystalline orientation phases. 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